Generation of Useful Insertionally Blocked Sterol Degradation Pathway Mutants of Fast-Growing Mycobacteria and Cloning, Characterization, and Expression of the Terminal Oxygenase of the 3-Ketosteroid 9 -Hydroxylase in Mycobacterium smegmatis mc155
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چکیده
10.1128/AEM.00941-06. 2006, 72(10):6554. DOI: Appl. Environ. Microbiol. Gábor Ambrus Jeanplong, Éva Ilkoy, Attila Kónya, István Kurucz and Attila Andor, Antónia Jekkel, David A. Hopwood, Ferenc 155 2 mc smegmatis Mycobacterium -Hydroxylase in α Terminal Oxygenase of the 3-Ketosteroid 9 Characterization, and Expression of the Fast-Growing Mycobacteria and Cloning, Sterol Degradation Pathway Mutants of Generation of Useful Insertionally Blocked
منابع مشابه
FadD19 of Rhodococcus rhodochrous DSM43269, a steroid-coenzyme A ligase essential for degradation of C-24 branched sterol side chains.
The actinobacterial cholesterol catabolic gene cluster contains a subset of genes that encode β-oxidation enzymes with a putative role in sterol side chain degradation. We investigated the physiological roles of several genes, i.e., fadD17, fadD19, fadE26, fadE27, and ro04690DSM43269, by gene inactivation studies in mutant strain RG32 of Rhodococcus rhodochrous DSM43269. Mutant strain RG32 is d...
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FABMS analysis of T-lb peptide before and after one cycle of Edman degradation indicated an unblocked N-terminal Thr residue for this tryptic peptide. In contrast , our data showed a molecular protonated ion, MH + for T- la peptide at 655 mass units (mu) which is 42 mu higher than the MH ion of T- 1b peptide. In addition, T- la peptide was not amenable to one cycle of manual Edman degrada...
متن کاملMycobacterium smegmatis is a suitable cell factory for the production of steroidic synthons
A number of pharmaceutical steroid synthons are currently produced through the microbial side-chain cleavage of natural sterols as an alternative to multi-step chemical synthesis. Industrially, these synthons have been usually produced through fermentative processes using environmental isolated microorganisms or their conventional mutants. Mycobacterium smegmatis mc2 155 is a model organism for...
متن کاملThe Role of fadD19 and echA19 in Sterol Side Chain Degradation by Mycobacterium smegmatis.
Mycobacteria are able to degrade natural sterols and use them as a source of carbon and energy. Several genes which play an important role in cholesterol ring degradation have been described in Mycobacterium smegmatis. However, there are limited data describing the molecular mechanism of the aliphatic side chain degradation by Mycobacterium spp. In this paper, we analyzed the role of the echA19...
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Objective: Fruits of lemon pepper (Zanthoxylum acanthopodium DC., Rutaceae) have been traditionally used as a spice and in folk medicine for treatment of diarrhea and stomachache. Stomachache could be associated with mycobacterial infection. The present study was designed to investigate the activity of Z. acanthopodium fruits against a non-infectious Mycobacterium smegmatis and to identify the ...
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